The Pellagra Portraits.

This Arts and Medicine feature reviews the history of pellagra and recounts the role of artist and illustrator John Carroll who, in 1919, painted portraits of people with the vitamin deficiency to document in color the appearance of pellagra skin plaques.

Posing biases in portraits of people that do not exist.

We report posing biases in portraits of people that do not exist. Studies of painted or photographed portraiture have often reported such biases. However, whether these truly exist or are mere sampling artifacts remains open to question. A novel approach to such a question is provided by contemporary applications generating photo-realistic virtual portraits. Such applications are exposed to large datasets of portraits of real people. A neural network then maps the variation of the original input set to a huge-dimensional generative model capturing the variation in the original data, which is then used to synthesize the virtual portraits. We reasoned that, if posing biases exist in the original input, they should also be observable in the network output, and they did. This finding provides novel support for the reality of posing biases in portraiture-and helps us better understand what generative networks actually do.

Engaging new audiences with imaging and microscopy.

In this Spotlight, we hear first-hand accounts from five scientists and educators who use microscopy and imaging to engage, entertain, educate and inspire new audiences with science and the field of developmental biology in particular. The 'voices' that follow each convey each authors' own personal take on why microscopy is such a powerful tool for capturing the minds, and the hearts, of scientists, students and the public alike. They discuss how microscopy and imaging can reveal new worlds, and improve our communication and understanding of developmental biology, as well as break down barriers and promote diversity for future generations of scientific researchers.

Editor Profile: Alexander Wlodawer.

In this special interview series, we profile members of The FEBS Journal editorial board to highlight their research focus, perspectives on the journal and future directions in their field. Alexander Wlodawer is Senior Investigator at the Center for Structural Biology at the National Cancer Institute Center for Cancer Research (NCI CCR), based in Maryland, USA. He has served as an editorial board member of The FEBS Journal since 2007.

The people behind the papers - Masaki Kinoshita, Toshihiro Kobayashi, Hiroshi Nagashima, Ramiro Alberio and Austin Smith.

The ability to derive and maintain pluripotent stem cells (PSCs) from livestock species in defined media conditions will contribute to many new research avenues, including comparative embryology and xenotransplantation. In a new paper in Development, Masaki Kinoshita, Toshihiro Kobayashi, Hiroshi Nagashima, Ramiro Alberio, Austin Smith and colleagues describe their three-component medium, which supports long-term propagation of PSCs in the absence of feeders or serum factors. We caught up with the authors to find out more about their research and their future plans.

What is in the black box? The discovery of the sigma factor and the subunit structure of E. coli RNA polymerase.

This Reflections article is focused on the 5 years while I was a graduate student (1964-1969). During this period, I made some of the most significant discoveries of my career. I have written this article primarily for a protein biochemistry audience, my colleagues who shared this exciting time in science, and the many scientists over the last 50 years who have contributed to our knowledge of transcriptional machinery and their regulation. It is also written for today's graduate students, postdocs, and scientists who may not know much about the discoveries and technical advances that are now taken for granted, to show that even with methods primitive by today's standards, we were still able to make foundational advances. I also hope to provide a glimpse into how fortunate I was to be a graduate student over 50 years ago in the golden age of molecular biology.

Identification of a Slovenian prewar elite couple killed in the Second World War.

Genetic identification of a Slovenian prewar elite couple killed in 1944 was performed by typing autosomal and Y-chromosomal STRs, and phenotypic HIrisPlex SNPs for hair and eye color prediction were analyzed for the female skeleton using next-generation sequencing (NGS) technology. The clandestine grave containing the couple's skeletal remains was found in 2015 and only the partial remains were found. Living distant relatives could be found only for the male victim. Because of a lack of comparative reference samples, it was not possible to identify the female victim through autosomal and mitochondrial DNA typing. However, the possibility of comparison of eye and hair color with a painting exhibited in the City Museum of Ljubljana by the prominent Slovenian painter Ivana Kobilca existed. Nuclear DNA obtained from the samples was quantified using the PowerQuant System, and then STR typing was carried out with different autosomal and Y-STR kits. From 0.09-9.36 ng DNA/g of powder was obtained from teeth and bones analyzed. Complete autosomal and Y-STR profiles made it possible to identify the male skeleton via comparison with two nephews. For the female victim, predicted eye and hair color was compared to colors on the painting. Kobilca's painting confirms the genetically predicted eye and hair color. After more than seventy years, the skeletal remains of the couple were handed over to their relatives, who buried the victims with dignity in a family grave.